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5 × 10 6 aspc-1 cells  (Charles River Laboratories)

 
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    Charles River Laboratories 5 × 10 6 aspc-1 cells
    Cancer cell killing effect and viral replication of oAd/RLX-PCDP complex-loaded hMSCs on various pancreatic cancer cells. (A) Oncolytic effect by PBS, naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. Cells <t>(AsPC-1,</t> PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded or oAd/RLX-PCDP-loaded hMSCs at various MOIs. After 4 (AsPC-1 and PANC-1) or 6 (MIA PaCa-2) days post-infection, cell viability was measured by MTT assay. For oAd/RLX groups, the cell viability solely accounts for the absorbance readout of respective cancer cell types. For oAd/RLX- and oAd/RLX-PCDP-loaded hMSC groups, the total cell viability (as determined by absorbance readout) accounts for both respective types of pancreatic cancer cells and surviving hMSC carrier. (B) Viral production. Cells (AsPC-1, PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. At 72 h post-infection, viral genomic copies were measured by real-time quantitative PCR. Results represent the mean ± SD of triplicate experiments. ***P < 0.001 versus oAd/RLX-loaded hMSC control.
    5 × 10 6 Aspc 1 Cells, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5+%C3%97+10+6+aspc-1+cells/aspc+1+cells/pmc06659406-128-16-28
    Average 90 stars, based on 1 article reviews
    5 × 10 6 aspc-1 cells - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration"

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    doi: 10.1016/j.jconrel.2019.04.040

    Cancer cell killing effect and viral replication of oAd/RLX-PCDP complex-loaded hMSCs on various pancreatic cancer cells. (A) Oncolytic effect by PBS, naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. Cells (AsPC-1, PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded or oAd/RLX-PCDP-loaded hMSCs at various MOIs. After 4 (AsPC-1 and PANC-1) or 6 (MIA PaCa-2) days post-infection, cell viability was measured by MTT assay. For oAd/RLX groups, the cell viability solely accounts for the absorbance readout of respective cancer cell types. For oAd/RLX- and oAd/RLX-PCDP-loaded hMSC groups, the total cell viability (as determined by absorbance readout) accounts for both respective types of pancreatic cancer cells and surviving hMSC carrier. (B) Viral production. Cells (AsPC-1, PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. At 72 h post-infection, viral genomic copies were measured by real-time quantitative PCR. Results represent the mean ± SD of triplicate experiments. ***P < 0.001 versus oAd/RLX-loaded hMSC control.
    Figure Legend Snippet: Cancer cell killing effect and viral replication of oAd/RLX-PCDP complex-loaded hMSCs on various pancreatic cancer cells. (A) Oncolytic effect by PBS, naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. Cells (AsPC-1, PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded or oAd/RLX-PCDP-loaded hMSCs at various MOIs. After 4 (AsPC-1 and PANC-1) or 6 (MIA PaCa-2) days post-infection, cell viability was measured by MTT assay. For oAd/RLX groups, the cell viability solely accounts for the absorbance readout of respective cancer cell types. For oAd/RLX- and oAd/RLX-PCDP-loaded hMSC groups, the total cell viability (as determined by absorbance readout) accounts for both respective types of pancreatic cancer cells and surviving hMSC carrier. (B) Viral production. Cells (AsPC-1, PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. At 72 h post-infection, viral genomic copies were measured by real-time quantitative PCR. Results represent the mean ± SD of triplicate experiments. ***P < 0.001 versus oAd/RLX-loaded hMSC control.

    Techniques Used: Infection, MTT Assay, Real-time Polymerase Chain Reaction

    Tumor-tropic migratory properties of oAd/RLX-PCDP complex-loaded hMSCs. oAd/RLX, oAd/RLX-PEI, and oAd/RLX-PCDP complex were treated into hMSCs at 5 MOI. After 18 h, these cells were detached and co-cultured with pancreatic cancer cells (AsPC-1) into the transwell plate for 30 h. Then, the migrated cells were fixed and stained with hematoxylin and eosin solution. All conditions were done in quadruplicate and repeated in two separate experiments. ***P < 0.001 versus uninfected hMSC (negative control) or naked oAd/RLX.
    Figure Legend Snippet: Tumor-tropic migratory properties of oAd/RLX-PCDP complex-loaded hMSCs. oAd/RLX, oAd/RLX-PEI, and oAd/RLX-PCDP complex were treated into hMSCs at 5 MOI. After 18 h, these cells were detached and co-cultured with pancreatic cancer cells (AsPC-1) into the transwell plate for 30 h. Then, the migrated cells were fixed and stained with hematoxylin and eosin solution. All conditions were done in quadruplicate and repeated in two separate experiments. ***P < 0.001 versus uninfected hMSC (negative control) or naked oAd/RLX.

    Techniques Used: Cell Culture, Staining, Negative Control

    In vivo antitumor efficacy of oAd/RLX-PCDP-loaded hMSCs in tumor-bearing mice. Pancreatic tumor xenograft model was established by injecting AsPC-1 cells (5 × 106) subcutaneously in nude mice (n=8). Following the confirmation of tumorigenesis, the treatments were systemically administered on 4 day intervals for total of 3 injections when the average tumor volumes reached 90 mm3. (A) Relative tumor volume of each treatment group at day 21 post treatment in respect to initial tumor volume from day 1. (B) Relative body weight of each treatment group in respect to PBS treatment group at 21 days post initial treatment. (C) Histological and immunohistochemical analysis of tumor tissues from mice treated with PBS, PCDP, hMSC, naked oAd/RLX, oAd/RLX-loaded hMSC, or oAd/RLX-PCDP-loaded hMSC. Tumor tissues were collected from mice at 3 days after the final treatment. Representative sections were stained with H & E or MT solution. TUNEL assay was performed to detect apoptosis, and the expression of E1A was assessed by immunohistochemistry. Data presented as mean ± SD. ***P < 0.001 versus PBS, PCDP, hMSC, naked oAd/RLX, and oAd/RLX-loaded hMSC.
    Figure Legend Snippet: In vivo antitumor efficacy of oAd/RLX-PCDP-loaded hMSCs in tumor-bearing mice. Pancreatic tumor xenograft model was established by injecting AsPC-1 cells (5 × 106) subcutaneously in nude mice (n=8). Following the confirmation of tumorigenesis, the treatments were systemically administered on 4 day intervals for total of 3 injections when the average tumor volumes reached 90 mm3. (A) Relative tumor volume of each treatment group at day 21 post treatment in respect to initial tumor volume from day 1. (B) Relative body weight of each treatment group in respect to PBS treatment group at 21 days post initial treatment. (C) Histological and immunohistochemical analysis of tumor tissues from mice treated with PBS, PCDP, hMSC, naked oAd/RLX, oAd/RLX-loaded hMSC, or oAd/RLX-PCDP-loaded hMSC. Tumor tissues were collected from mice at 3 days after the final treatment. Representative sections were stained with H & E or MT solution. TUNEL assay was performed to detect apoptosis, and the expression of E1A was assessed by immunohistochemistry. Data presented as mean ± SD. ***P < 0.001 versus PBS, PCDP, hMSC, naked oAd/RLX, and oAd/RLX-loaded hMSC.

    Techniques Used: In Vivo, Immunohistochemical staining, Staining, TUNEL Assay, Expressing, Immunohistochemistry

    Related Articles

    In Vivo:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    Infection:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    MTT Assay:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    Real-time Polymerase Chain Reaction:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    Cell Culture:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    Staining:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    Negative Control:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    Immunohistochemical staining:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    TUNEL Assay:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    Expressing:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    Immunohistochemistry:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).

    Modification:

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration
    Article Snippet: hMSCs were seeded at a density of 5 × 10 3 cells/well in a 96-well ImageLock plate (Essen BioScience, Ann Arbor, MI).



    Similar Products

    5 × 10 6 aspc-1 cells  (Charles River Laboratories)
    90
    Charles River Laboratories 5 × 10 6 aspc-1 cells
    Cancer cell killing effect and viral replication of oAd/RLX-PCDP complex-loaded hMSCs on various pancreatic cancer cells. (A) Oncolytic effect by PBS, naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. Cells <t>(AsPC-1,</t> PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded or oAd/RLX-PCDP-loaded hMSCs at various MOIs. After 4 (AsPC-1 and PANC-1) or 6 (MIA PaCa-2) days post-infection, cell viability was measured by MTT assay. For oAd/RLX groups, the cell viability solely accounts for the absorbance readout of respective cancer cell types. For oAd/RLX- and oAd/RLX-PCDP-loaded hMSC groups, the total cell viability (as determined by absorbance readout) accounts for both respective types of pancreatic cancer cells and surviving hMSC carrier. (B) Viral production. Cells (AsPC-1, PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. At 72 h post-infection, viral genomic copies were measured by real-time quantitative PCR. Results represent the mean ± SD of triplicate experiments. ***P < 0.001 versus oAd/RLX-loaded hMSC control.
    5 × 10 6 Aspc 1 Cells, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5+%C3%97+10+6+aspc-1+cells/aspc+1+cells/pmc06659406-128-16-28
    Average 90 stars, based on 1 article reviews
    5 × 10 6 aspc-1 cells - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Cancer cell killing effect and viral replication of oAd/RLX-PCDP complex-loaded hMSCs on various pancreatic cancer cells. (A) Oncolytic effect by PBS, naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. Cells (AsPC-1, PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded or oAd/RLX-PCDP-loaded hMSCs at various MOIs. After 4 (AsPC-1 and PANC-1) or 6 (MIA PaCa-2) days post-infection, cell viability was measured by MTT assay. For oAd/RLX groups, the cell viability solely accounts for the absorbance readout of respective cancer cell types. For oAd/RLX- and oAd/RLX-PCDP-loaded hMSC groups, the total cell viability (as determined by absorbance readout) accounts for both respective types of pancreatic cancer cells and surviving hMSC carrier. (B) Viral production. Cells (AsPC-1, PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. At 72 h post-infection, viral genomic copies were measured by real-time quantitative PCR. Results represent the mean ± SD of triplicate experiments. ***P < 0.001 versus oAd/RLX-loaded hMSC control.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration

    doi: 10.1016/j.jconrel.2019.04.040

    Figure Lengend Snippet: Cancer cell killing effect and viral replication of oAd/RLX-PCDP complex-loaded hMSCs on various pancreatic cancer cells. (A) Oncolytic effect by PBS, naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. Cells (AsPC-1, PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded or oAd/RLX-PCDP-loaded hMSCs at various MOIs. After 4 (AsPC-1 and PANC-1) or 6 (MIA PaCa-2) days post-infection, cell viability was measured by MTT assay. For oAd/RLX groups, the cell viability solely accounts for the absorbance readout of respective cancer cell types. For oAd/RLX- and oAd/RLX-PCDP-loaded hMSC groups, the total cell viability (as determined by absorbance readout) accounts for both respective types of pancreatic cancer cells and surviving hMSC carrier. (B) Viral production. Cells (AsPC-1, PANC-1, and MIA PaCa-2) were infected with naked oAd/RLX, oAd/RLX-loaded hMSCs, or oAd/RLX-PCDP-loaded hMSCs. At 72 h post-infection, viral genomic copies were measured by real-time quantitative PCR. Results represent the mean ± SD of triplicate experiments. ***P < 0.001 versus oAd/RLX-loaded hMSC control.

    Article Snippet: In vivo antitumor efficacy Pancreatic cancer xenograft model was established by injecting 5 × 10 6 AsPC-1 cells into the subcutaneous of 6 weeks-old female nude mice (n=8) (Charles River Inc., Wilmington, MA).

    Techniques: Infection, MTT Assay, Real-time Polymerase Chain Reaction

    Tumor-tropic migratory properties of oAd/RLX-PCDP complex-loaded hMSCs. oAd/RLX, oAd/RLX-PEI, and oAd/RLX-PCDP complex were treated into hMSCs at 5 MOI. After 18 h, these cells were detached and co-cultured with pancreatic cancer cells (AsPC-1) into the transwell plate for 30 h. Then, the migrated cells were fixed and stained with hematoxylin and eosin solution. All conditions were done in quadruplicate and repeated in two separate experiments. ***P < 0.001 versus uninfected hMSC (negative control) or naked oAd/RLX.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration

    doi: 10.1016/j.jconrel.2019.04.040

    Figure Lengend Snippet: Tumor-tropic migratory properties of oAd/RLX-PCDP complex-loaded hMSCs. oAd/RLX, oAd/RLX-PEI, and oAd/RLX-PCDP complex were treated into hMSCs at 5 MOI. After 18 h, these cells were detached and co-cultured with pancreatic cancer cells (AsPC-1) into the transwell plate for 30 h. Then, the migrated cells were fixed and stained with hematoxylin and eosin solution. All conditions were done in quadruplicate and repeated in two separate experiments. ***P < 0.001 versus uninfected hMSC (negative control) or naked oAd/RLX.

    Article Snippet: In vivo antitumor efficacy Pancreatic cancer xenograft model was established by injecting 5 × 10 6 AsPC-1 cells into the subcutaneous of 6 weeks-old female nude mice (n=8) (Charles River Inc., Wilmington, MA).

    Techniques: Cell Culture, Staining, Negative Control

    In vivo antitumor efficacy of oAd/RLX-PCDP-loaded hMSCs in tumor-bearing mice. Pancreatic tumor xenograft model was established by injecting AsPC-1 cells (5 × 106) subcutaneously in nude mice (n=8). Following the confirmation of tumorigenesis, the treatments were systemically administered on 4 day intervals for total of 3 injections when the average tumor volumes reached 90 mm3. (A) Relative tumor volume of each treatment group at day 21 post treatment in respect to initial tumor volume from day 1. (B) Relative body weight of each treatment group in respect to PBS treatment group at 21 days post initial treatment. (C) Histological and immunohistochemical analysis of tumor tissues from mice treated with PBS, PCDP, hMSC, naked oAd/RLX, oAd/RLX-loaded hMSC, or oAd/RLX-PCDP-loaded hMSC. Tumor tissues were collected from mice at 3 days after the final treatment. Representative sections were stained with H & E or MT solution. TUNEL assay was performed to detect apoptosis, and the expression of E1A was assessed by immunohistochemistry. Data presented as mean ± SD. ***P < 0.001 versus PBS, PCDP, hMSC, naked oAd/RLX, and oAd/RLX-loaded hMSC.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration

    doi: 10.1016/j.jconrel.2019.04.040

    Figure Lengend Snippet: In vivo antitumor efficacy of oAd/RLX-PCDP-loaded hMSCs in tumor-bearing mice. Pancreatic tumor xenograft model was established by injecting AsPC-1 cells (5 × 106) subcutaneously in nude mice (n=8). Following the confirmation of tumorigenesis, the treatments were systemically administered on 4 day intervals for total of 3 injections when the average tumor volumes reached 90 mm3. (A) Relative tumor volume of each treatment group at day 21 post treatment in respect to initial tumor volume from day 1. (B) Relative body weight of each treatment group in respect to PBS treatment group at 21 days post initial treatment. (C) Histological and immunohistochemical analysis of tumor tissues from mice treated with PBS, PCDP, hMSC, naked oAd/RLX, oAd/RLX-loaded hMSC, or oAd/RLX-PCDP-loaded hMSC. Tumor tissues were collected from mice at 3 days after the final treatment. Representative sections were stained with H & E or MT solution. TUNEL assay was performed to detect apoptosis, and the expression of E1A was assessed by immunohistochemistry. Data presented as mean ± SD. ***P < 0.001 versus PBS, PCDP, hMSC, naked oAd/RLX, and oAd/RLX-loaded hMSC.

    Article Snippet: In vivo antitumor efficacy Pancreatic cancer xenograft model was established by injecting 5 × 10 6 AsPC-1 cells into the subcutaneous of 6 weeks-old female nude mice (n=8) (Charles River Inc., Wilmington, MA).

    Techniques: In Vivo, Immunohistochemical staining, Staining, TUNEL Assay, Expressing, Immunohistochemistry